#Calcusyn biosoft software#
Furthermore, eupneria J and eurifoloid H showed significant anti-HIV-1 activities with IC 50 values of 0.31 and 6.70 μg/mL, respectively, and ent-isopimara-8(14),15-dien-3 β,12 β-diol possessed obvious anti-influenza virus activity against A/Puerto Rico/8/1934, with an IC 50 at 3.86 μg/mL. A more detailed description of degrees of synergism and antagonism is adapted from Chou and Hayball (CalcuSyn, Windows software for dose effect analysis. Results: Mean IC50s of drugs alone and in combination are. Cell nuclei and cytoskeleton staining assay Cells were seeded on glass cover slips in 6-well plates. Analysis of combined effects was performed using the median effect principle (CalcuSyn, Biosoft).
The detailed description on the combi-nation is provided in the Supplementary Materials. A CI less than 0.9 was dened as synergism. In addition, the absolute configuration of the known compound, 3 β-hydroxysandaracopimaric acid, was determined by comparing its ECD spectrum with eupneria J, and renamed as eupneria P. Calcusyn(Biosoft).Thecombinationindex(CI)wascalculated by the ChouTalalay equation (11). The absolute configuration of eupneria J was established based on the X-ray diffraction analysis and the experimental and calculated electronic circular dichroism (ECD), while the absolute configuration of eupnerias K–N were determined by the experimental and calculated ECD. These include programs for data analysis, graph plotting, statistics, densitometry, dose-effect analysis, assays, curve-fitting, enzyme kinetics, teaching and more. Structurally, eupnerias J−M were the first examples of 18 (or 19)-norditerpenoid with ent-isopimarane skeleton from E. BIOSOFT sells Windows based scientific software packages for a wide range of purposes. For drug combination studies, drug dilutions were performed based on fixed 1:1 ratio of IC 50 values for ixazomib and belinostat, and assessment MTT-based cell viability at 72 h. (a) Calcusyn analysis of gercumin + FOLFOX synergy in CR-HT29 cells. Western blot analysis was performed as reported previously 25. The software program Calcusyn (Biosoft, Ferguson, MO) was employed to determine the.
Experimental values were imputed into Calcusyn to calculate IC50 and a combination index (CI, a quantitative measure of the synergy (CI < 1), additivity (CI 1), or antagonism (CI > 1) between drugs).Twelve ent-isopimarane diterpenoids, including six undescribed ones, eupnerias J−O, were isolated from the stem barks of Euphorbia neriifolia L. IC 50 and combination indices for these assays were determined using method of Chou and Talalay and Calcusyn (Biosoft, Ferguson, MO, USA). values were determined using CalcuSyn software (Biosoft. Drug combinations were evaluated using CalcuSyn (BIOSOFT, Ferguson, MO) software based on the multiple drug effect equation of Chou-Talalay.
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